In the present study physical, chemical and microbiological characteristics of the water from different sites of Rajkot, Gujarat (India). Diseases related to contamination of drinking water constitute a major problem on human health. Intervention to improve the quality of drinking water provides significant benefits to health. Therefore, in this study 30 samples were collected. The various Physical and Chemical parameters studied were Odor, Taste, pH, TDS, TSS, Total Hardness, Calcium, Sulfate, Nitrate and Phosphate. The result indicated that physical parameters i.e., Odor, Taste and pH were within the permissible limit. In the Total Hardness most of the samples are within the portability limit. The concentration of Calcium, Magnesium, Chloride and Nitrate in some samples was high and concentration of Sulfate and Phosphate were within the portability limit. In our investigation it was found that almost all sources were positive for E.coli, Salmonella and Shigella differential analysis. This investigation revealed that all most samples were not completely fit for drinking purpose with respect to Physical, Chemical and microbiological parameters studied.